Microscopy from Scientific Research

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NCI-H1975 WT or monoclonalTMEM106BKOcells, incubated with SARS-CoV-2 at MOI 8 on ice, followed by virus internalization at 35°C for 0 or 2 h in the presence of 20 μg/mL cycloheximide to block translation. Cells were stained for nucleocapsid before permeabilization (green) and after permeabilization (red) and nuclei (blue). Shown are representative images, scale bars, 10 μm. A magnification of the area in the square is shown in each upper right corner.
#Microscopy#NCI-H1975 WT cells#Monoclonal TMEM106B KO cells#SARS-CoV-2#Cycloheximide#Nucleocapsid#Immunofluorescence
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