Gel from Scientific Research

Open access visualization of Gel, FLAG-VAPB, HA-Ubiquitin, GFP, GFP-VAPB
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DOI: 10.1038/s41421-023-00576-6

DOI

FLAG-VAPB and HA-ubiquitin were co-transfected with GFP or GFP-VAPB into C2C12 myoblasts with the indicated combination for 36 h. After 5 h of MG132 (20 μM) treatment, IRS-1 ubiquitination was determined by immunoprecipitation with a FLAG antibody and immunoblotted with an HA antibody.

#Gel#FLAG-VAPB#HA-Ubiquitin#GFP#GFP-VAPB#C2C12 Myoblasts#MG132#IRS-1 Ubiquitination#Immunoprecipitation

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