In vitro phosphorylation of MBP a by ZmWAKL Y -GFP and ZmWAKL Q -GFP. ZmWAKL Y -GFP and ZmWAKL Q -GFP were separately immunoprecipitated from transgenic plants overexpressing ZmWAKL Y -GFP or ZmWAKL Q -GFP . Phosphorylated MBP a was detected by autoradiography (autorad), and the protein loading is shown by CBB staining. ZmWAKL-GFP was detected with -GFP antibody, with plant actin as a loading control. The Co-IP, SLC assays and phosphorylation assays are repeated at least two times.
