Proportion of in vitro Micro-C read numbers clustered by the orientation of the interacting nucleosomes, as indicated in the scheme on the right. Chromatin used for in vitro Micro-C was incubated with the indicated remodeler and the TFs Abf1 and Reb1 or with the TFs only (control). For comparison, in vivo Micro-C 13 and Hi-CO 33 data are shown.