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XCR1-DTR mice were pre-immunized i.v. with OVA GLP. At day 20, half the mice were treated with DT to deplete cDC1. The following day, 1 x 105 naive CTV-labeled OT-I cells were transferred into vaccinated mice and OT-I cell proliferation was examined by flow cytometry in the spleen 10 days later. NIL indicates unvaccinated mice that received naive CTV-labeled OT-I cells. Circles represent individual mice. Data are pooled from two to four independent experiments (n= 4–9 mice per group for each time point) except for day 21 with only one experiment (n= 2 mice).
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