Illustration from Scientific Research

Schematic of a multispecific antibody chassis variant library created from a set of antibodyDNA conjugates. The symbols indicate the antibody, and the colour indicates the engaged cell type. Antibodies are covalently tagged with DNA handles with the sequences A, B, C or D, depending on the library, and the sequences are complementary to DNA handles on the chassis (centre). The chassis carries four DNA handles. Antibody chassis variants are produced by mixing the respective antibodies from the libraries with the DNA chassis. Variants are named by their antibody combination (the centre bottom shows an example combination). Two reference-free class averages calculated from single-particle TEM micrographs. Scale bar, 20 nm. The top average shows the platform without antibodies and the bottom average image shows the platform with four IgG antibodies, indicated by the orange and blue arrow heads pointing to the blurred additional signal in the average image.

DOI