Barplot showing percentage viability of K562 48 h after co-culture with healthy donor PBCMs at an effector:target ratio of 10:1 with or without the respective TCEs (0.1 nM) or BiTE (5.4 nM) as indicated. Co-culture with anti-BCMA CAR T was performed at an effector:target ratio of 1:1. The x axis corresponds to the experimental conditions. The samples shown are biologically independent for K562 alone ( n = 4), K562 + PBMCs ( n = 4), K562 + PBMCs + TEC ( n = 4), K562 + PBMCs + ERLA ( n = 4), K562 + PBMCs + BiTE ( n = 3) and K562 + PBMCs + CAR T ( n = 3). The Student’s t -test was performed on each grouped sample (each K562 cell line) without adjustments for multiple comparisons using the R function pairwise.t.test() to generate P values. Absolute P values are provided in Supplementary Table 8 . Data are presented as mean ± s.d.
