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Schematic drawing of the in vivo Perturb-seq: LSK progenitors were sorted from Cas9-GFP mice infected with the chromatin factor knockout library and transplanted into irradiated recipient mice; bone marrow was collected 14 days after transplant, sorted for an immature phenotype (lineage - and Lin + c-Kit + ) and the Perturb-seq and downstream analyses were performed.

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