DLA with 35S :: Rpi-amr4-1102 . 35S :: Rpi-amr4-1102 (green), Rpi-amr3 (positive control, red) and Rpi-amr3a (a non-functional Rpi-amr3 paralog, negative control, blue) were transiently expressed in N. benthamiana, OD 600 = 0.5. Zoospores from P. infestans strain T30-4 were used to inoculate the leaves 1 day post-infiltration (dpi). Lesion sizes were measured at 6 dpi. Four biological replicates were performed, and all data points (74 data points per treatment) were visualized as a box-and-whisker plot. Center line, median; box limits, upper and lower quartiles. The whiskers (top and bottom) comprise values within 1.5 times the interquartile range (IQR). The outliers are indicated by black dots. Statistical differences were analyzed by one-way ANOVA with Tukey’s HSD test (P < 0.001) and were indicated by the lower-case letters. Representative leaves are shown.
