Photo from Scientific Research

HR assay of candidate genes. Rpi-amr4-1102 and Rpi-amr4-2271 were expressed alone or co-expressed with either 35S::PITG_22825 or 35S::AVRamr3-GFP constructs in N. benthamiana leaves. Rpi-amr4-2271 is auto-active in N. benthamiana, but when co-expressed with PITG_22825, the HR was stronger. Rpi-amr4-1102 specifically recognizes PITG_22825. Rpi-amr3 was used as control. OD 600 = 0.5. Four leaves from two plants were used for each experiment and three biological replicates were performed with the same results. HF, HisFlag.

DOI