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Rpi-amr4 -knockout lines lose the capacity for PITG_22825 recognition. Two sgRNAs (black arrows) were designed on Rpi-amr4 - 2271 . The genotype of the two knockout lines is shown. Both lines failed to recognize PITG_22825, but HR could be complemented when co-expressing PITG_22825 with Rpi-amr4-1102 . Wild-type (WT) SP2271 plants were used as control plants. Rpi-amr3 and AVRamr3 were used as positive control. OD 600 = 0.5.

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