A total of 315 RXLR effectors were transiently expressed in 52 S. americanum accessions. The HR index (2, strong HR; 1, weak HR; 0, no HR, NA, not available) was used for the heatmap. These effectors were screened on N. benthamiana 30 , and their recognitions are included in this heatmap. Empty PVX vector was used as negative control, and co-expression of Rpi-amr3HisFlag and AVRamr3GFP was used as positive control. The S. americanum accessions were ordered based on the phylogenetic tree; SP3400 is not included in this tree. The scale bar represents the number of amino acid substitutions per site. The S. americanum accessions were classified into four groups (gray or yellow shading). The four reference accessions are marked by red arrows. The effectors were ordered based on the total HR index. For each effector, the numbers of responsive S. americanum accessions is visualized by a bar chart on the top of the heatmap. For each S. americanum accession, the numbers of recognized effectors is visualized by a bar chart on the right of the heatmap. Some RXLR effectors previously characterized or mentioned in this study are indicated by gray arrows.
