The NB-ARC domains of S. americanum SP1102 were predicted by NLR-annotator and used to generate a maximum-likelihood tree using IQ-TREE with the JTT + F + R9 model. Known NLR proteins from Solanaceae species were included (highlighted in red). The NLRs are classified into different groups based on a previous report 29 . The RNL, TNL and NRC superclade are shown. CED-4 from Caenorhabditis elegans was used as the outgroup. The expression profile is shown by a heatmap (white to red) based on the cDNA RenSeq data of SP1102. The P/A polymorphism of NLRs from the three other S. americanum genomes and SMRT RenSeq assemblies of 16 additional accessions are shown by the heatmap (white to blue). The accession order from top to bottom is SP3409, SP3408, SP3406, SP3400, SP3399, SP3370, SP2360, SP2308, SP2307, SP2300, SP2298, SP2272, SP1123, SP1101, SP1034, SP1032, SP2275, SP2273 and SP2271. The absent NLRs are shown by black blocks. The scale bar represents the number of amino acid substitutions per site.
