Comparison of editing efficiencies for four different prime edits targeting HEK4 locus in HEK293T cells following plasmid transfection or treatment with v3b PE-eVLPs at the on-target HEK4 site and known off-target sites for the corresponding pegRNA 1 . Values shown in all graphs and heatmaps represent the average prime editing efficiency of three biological replicates, and error bars represent the standard deviation. Data were fitted to four-parameter logistic curves using nonlinear regression. For all conditions other than f , 30,00035,000 cells were treated with eVLPs containing ~2.5 10 8 eVLPs μl 1 .
