Schematic of v3 PE-eVLPs utilizing the MCPMS2 strategy for the recruitment of epegRNAs. The incorporation of MCPMS2 strategy enables three modes of guide RNA loading into eVLPs: (i) via binding to PE, (ii) via MS2 stemloop binding to MCP and (iii) via MCP:MS2gRNAs:PE threecomponent interaction.
