Microscopy from Scientific Research

Single xy and xz planes of four example super-resolved volumes comprising specific synapses as marked in a , with coCATS (gray, z -STED, STAR RED-NHS, N2V) revealing local microenvironment of the positively labeled mossy fiber (yellow, z -STED, N2V) (raw data: Supplementary Fig. 4 ). Bottom: magnified views of the coCATS channel with asterisks indicating pSCRs used to identify synaptic partners. pSCRs were segmented with the same model as in Fig. 2j,k , followed by manual proofreading.

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