ChIP-qPCR for the indicated proteins ( left ) at the TetO array inserted into mESCs expressing the indicated TetR fusion proteins ( top ). Dashed line shows ChIP-qPCR in cells treated with doxycycline for 6 h. Data are represented as mean SEM, n = 4 independent experiments for TetR FS2 in TetR, TetR FS2 in TetR-CXXC, TetR FS2 in TetR-Paf1, Enl in TetR, Enl in TetR-ENL, Paf1 in TetR, Paf1 in TetR-PAF1; n = 3 independent experiments for TetR FS2 in TetR-ENL, TetR FS2 in TetR-DOT1L, Enl in TetR-CXXC, Enl in TetR-Paf1, Paf1 in TetR-CXXC, Paf1 in TetR-ENL, Ssrp1 in TetR, Ssrp1 in TetR-CXXC, Ssrp1 in TetR-ENL, Ssrp1 in TetR-PAF1 and n = 2 independent experiments for Enl in TetR-DOT1L, Paf1 in TetR-DOT1L, Ssrp1 in TetR-DOT1L. Source data are provided as a Source Data file.
