Microscopy from Scientific Research

IHC staining of junctional protein ZO-1 in 73 C GEMM at 7–21 dpi. The blood vessels are stained with CD31 (red), and the cell nuclei are indicated by Hoechst staining (HOE, blue). The arrows indicate blood vessels, and the arrowheads indicate tight junction proteins. The scale bars represent 20 m. The quantification of ZO-1 coverage on the blood vessel by area fraction. N = 15 images from 3 mice. Data were analyzed by One-way ANOVA followed by Tukey’s multiple comparisons test, n.s. represents no significant difference. Source data are available as a Source Data file.

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