TRAIP-mAID cells were arrested in G2 stage of the cell cycle and TRAIP degraded before release of cells into mitosis. After release auxin (IAA) was optionally washed off 3 h after release, so that cells progressed through next S-phase with or without TRAIP. Cell-cycle progression was analysed by FACS at 24 h post release from G2 arrest. All analyses presented as in A . Any difference between treatment groups was determined using one-way ANOVA and post hoc testing, significance is summarised on the plot (one-way ANOVA: p < 0.0001; Tukey’s post hoc testing, TRAIP-mAID C2: −IAA vs +IAA, p < 0.001; −IAA vs +IAA WASH, p = 0.800; +IAA vs +IAA WASH, p < 0.001. TRAIP-mAID D5: −IAA vs +IAA, p = 0.00107; −IAA vs +IAA WASH, p = 0.898; +IAA vs +IAA WASH, p = 0.00554). Source data are provided as a Source Data file.
