Bar Plot from Scientific Research

TRAIP-mAID cells were grown in optional presence of auxin (IAA) for 24 h and stained with markers of DNA damage response (γH2AX and 53BP1). Example of immunofluorescent visualisation of DNA damage (left) and quantification of percentage of cells containing increasing numbers of γH2AX and 53BP1 foci over 3 independent experiments (right). Significance testing carried out using one-way ANOVA and subsequent Tukey’s post hoc testing (TRAIP-mAID C2: ANOVA – p < 0.001; Tukey’s post hoc test − 0 Foci: p = <0.001; 1–4 Foci: p < 0.001; 5-9 Foci: p = 0.0168; 10 + Foci: p = 0.0059. TRAIP-mAID D5: ANOVA – p < 0.001; Tukey’s post hoc test − 0 Foci: p < 0.001; 1–4 Foci: p < 0.001; 5–9 Foci: p = 0.0046. Source data are provided as a Source Data file.

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