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LiGA was injected (tail vein) into mice ( n = 3 animals). After 1 h, mice were perfused with saline and euthanized. Plasma and organs were collected; organ homogenates and plasma were incubated with E. coli for 3 h to amplify the phage, followed by PCR and NGS of phage DNA. Fold change (FC) between organ and plasma was calculated by edgeR after normalizing by scaling the unmodified phages to unity, and multi-test control by FDR from B-H correction.

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