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Time-course analysis of Gd III -triggered SDRs. The reaction was started by the addition of Gd III ions (4 equiv). In the control experiment, strands 7t and 8t , in which U OH bases are replaced with natural T bases, were used instead of strands 7 and 8 . The toehold-mediated reaction was carried out with FAM-labeled 7t, 8 s (21-mer that lacks the terminal 5′-G U OH U OH U OH U OH -3′), and Dabcyl-labeled 9 .
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