Workflow from Scientific Research

KARR-seq workflow. Cells are first treated with N3-kethoxal to modify RNAs with azide tags (red), which enables crosslinking of the tagged RNA molecules by DBCO-decorated dendrimers (blue). Biotin modifications (pink) on dendrimers facilitate the enrichment of crosslinking products, followed by proximity ligation, RNA library construction and sequencing. Chimeric sequencing reads are aligned to identify RNA–RNA interactions.

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