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Our study commences with the 3-week rhEPO (N = 6) and PL (N = 6) treatments in male C57BL/6 mice. After the last injection, we processed 12 samples, 6 each from EPO and PL mice, each a pool of 2 right hippocampi (i.e., a pool of 2 mice). These samples were then subjected to snRNA-seq, and the resulting data were strategically analyzed to investigate the molecular, pseudo-temporal, and empirical changes that occur in pyramidal lineages following EPO treatment. Finally, we performed a series of single-cell electrophysiology experiments to demonstrate that EPO affects excitatory and inhibitory input to newly formed and pre-existing pyramidal neurons in mouse hippocampi. Drop-seq design created with BioRender.com.

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