Microscopy from Scientific Research

MINFLUX imaging and PIEZO1 trimer identification. Representative x100 confocal scan (from n = 5 cells) of a HEK293 cell membrane expressing labelled TCO*K 103 PIEZO1 (scale bar, 2 µm) (left). Colour represents scaled intensity in each detector (650–685 nm (green) and 685–720 nm (red)). Clusters of raw localizations (traces) rendered as 15-nm spheres from the same region (scale bar, 2 µm) (middle). Zoomed-in rendering (3-nm spheres) showing single-labelled (red), double-labelled (yellow) and triple-labelled (green) PIEZO1 identified by the algorithm (scale bar, 20 nm) (right).

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