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Primary and secondary xenotransplantation of FLT3 N399 or AAVS1 BE HSPCs. Top, human engraftment (hCD45 + ) by flow cytometry analysis in primary recipients. Data are mean s.d. Statistical analysis was performed using two-way ANOVA. n = 7 ( FLT3 N399 ) and n = 4 ( AAVS1 BE ). Bottom, FLT3 editing was measured on peripheral blood, haematopoietic organs (BM and spleen (SP)) or CFUs in primary and secondary transplants. Data are mean s.d. Statistical analysis was performed using one-way ANOVA. LC, liquid culture; SP, spleen; W, week.

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