Scatter Plot from Scientific Research

FLT3 N399D or FLT3 N399G cells avoid 4G8-CAR-mediated killing. K562 cells expressing FLT3 variants were cultured with 4G8-CAR at different effector:target ratios (E:T). Left, FACS analysis of K562 cells expressing either FLT3 WT, N399D or N399G, after 48 h of co-culture. T cells and targets are identified by CD3 and FLT3, respectively. From left to right, the fraction of live target cells (absolute (abs.) counts of annexin V - 7-AAD - cells) relative to E:T = 0; T cell activation by CD69 + (%) and surface expression of FLT3 by BV10A4 staining on residual live targets, normalized (norm.) to E:T = 0. The E:T ratio is reported on the x axis. Data are mean s.d. n = 4. Statistical analysis was performed using two-way analysis of variance (ANOVA); P values of the comparisons between each condition are reported.

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