Glucagon-induced GCGR–βarr1 interaction for the GCGR and βarr1 mutants measured by BRET assays. ΔFL, the βarr1 mutant with the turn region of the finger loop (residues 66–73) removed. The EC 50 ratios, EC 50 (mutant)/EC 50 (wild type), are represented by bars. Extended Data Table 1 provides detailed independent experiment numbers ( n ), statistical evaluation, P values and expression levels. A schematic of the GCGR–βarr complex in the tail conformation is also shown (right). The biosensors labelled in GCGR and βarr that were used in the BRET assay are indicated by blue and green stars, respectively. ND, not determined.
