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ZDHC15 substrate discovery in HEK293T cells treated with 15 M 20- c Pr in HEK293T cells using the OBH workflow. In total, 107 chemicalgenetic ZDHHC15 substrates were identified (Students two-tailed unpaired t test, S 0 = 0.5, adjusted FDR = 0.01, n = 4 independent biological replicates). Substrates unique or in common with parallel analyses for DHHC7 and DHHC20 in HEK293T cells are highlighted (Extended Data Fig. 10).
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