Gel from Scientific Research

Open access visualization of Gel, HA-VAMP3 S-Acylation, HA-BCAP31 S-Acylation, ZDHHC20, 18-Bz Bumped Probe
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DOI: 10.1038/s41587-023-02030-0

DOI

Validation of HA-VAMP3 and HA-BCAP31 site S -acylation by ZDHHC20 using the bumped probe 18-Bz and S -acylation site mutants, VAMP3(C76A) and BCAP31(C23A). Cell-based transfer assays were performed without FLAG-ZDHHC20 and HA-VAMP3 enrichment, but rather with direct labeling of cell lysates by TAMRA-azide click followed by SDS–PAGE and anti-HA, anti-FLAG and anti-vinculin immunoblot analysis.

#Gel#HA-VAMP3 S-Acylation#HA-BCAP31 S-Acylation#ZDHHC20#18-Bz Bumped Probe#S-Acylation Site Mutants#Cell-Based Transfer Assays#TAMRA-Azide Click#SDS-PAGE#Anti-HA Immunoblot#Anti-FLAG Immunoblot#Anti-Vinculin Immunoblot

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