Left, normalized to minimum FRET ratio timecourses of Beas2B cells transfected with Ras-LOCKR-S localized to EML4-Alk v1 and incubated with 10 M of the inhibitors shown ( n = 21 cells per condition). Puncta and diffuse regions were analyzed separately (** P = 0.0022 and **** P = 1.6 10 -18 ). Normalized to minimum FRET ratios are calculated by normalizing the dataset to the condition with the largest decrease in FRET ratios (Ce + Y in both cases), where 0 represents the lowest FRET ratio out of the entire dataset. Right, raw FRET ratios of puncta and diffuse EML4-Alk regions after Alk inhibition for 1 h. Bar graphs represent mean s.e.m. **** P < 0.0001 and ** P < 0.01, one-way ANOVA. Scale bars, 10 m.
