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Maximum likelihood phylogeny of dactynotine aphids based on a concatenated alignment of 5091 conserved single-copy protein coding genes. We included seven outgroup aphid species from Aphidinae but for simplicity only show M. persicae . The full phylogeny is shown in Additional File 2 : Figure S11. All branches received maximal support according to the Shimodaira-Hasegawa test [ 52 ] implemented in FastTree [ 53 , 54 ] with 1000 resamples. Branch lengths are in amino acid substitutions per site. The tree is annotated with genome assembly length and gene counts coloured by orthology relationships across the full phylogeny.

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