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The levels of IgG specific to B. afzelii proteins were measured by semi-quantitative ELISA in pre-adsorbed sera of B. afzelii -infected mice immunized with E. coli , non-pre-adsorbed E. coli + B. afzelii or PBS + B. afzelii sera. One-way ANOVA with Tukeys multiple comparisons test (*** p < 0.001; 4 technical replicates per sample).
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