Line Plot from Scientific Research

BS reaction of an RNA probe (5′-AGCGA) was monitored by MALDI-TOF MS. The peaks at 1,592, 1,675 and 1,593 were assigned to RNA oligo probes containing unconverted C, U-BS and final product U, respectively. As comparison, the RNA probe containing a m 5 C modification (5′-AGm 5 CGA) showed no visible reaction after 10 min of UBS-2 treatment. The peak at 1,606 represented unreacted m 5 C-containing oligo probe, and no peak representing the corresponding product was observed at 1689.

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