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Fecal pellets were collected from HFD mice at 8 weeks and then used to perform fecal microbiota transplantation (FMT). Standard diet-fed mice were treated with an antibiotic cocktail for 3 days, followed by FMT lasting 4 weeks (C-FMT). HFD-fed mice were concurrently treated with an antibiotic cocktail for 4 weeks (H-Abx) to further verify the critical role of gut microbiota in diet-mediated disruption of tryptophan (Trp)-kynurenine (Kyn) metabolism.

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