Box Plot from Scientific Research

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Boxplot showing viral copy numbers (log10 transformed) of RG-patients and KR-patients (two-tailed t -test, KR-patients versus RG-patients, p -value < 0.0001 (****)). The copy numbers were calculated using standard curve from Ct values (RT-qPCR) of the N1 primer pairs after normalization with RNase P Ct values. Extracted RNA (by Direct-Zol kit, Zymo Research) was subjected to RT-qPCR using one-step TaqPath kit (Applied Biosystems) and nucleocapsid gene (N1) and RNAseP primers.
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