Gel from Scientific Research

Representative plasmid-based activity assay of D7 and D7-ZF on predicted human genomic loxF8-like off-targets flanked with the sequences potentially recognized by D7-ZF. For b – d , tests were performed at high induction level (100 µg ml −1 L-arabinose). The upper band represents the unrecombined plasmid (line with two triangles); the lower band represents the recombined plasmid (line with one triangle). M, marker. Activity of D7 is shown as a control. The assays were performed three times ( n = 3, biologically independent samples; replicates are shown in Source Data files).

DOI