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Schematic figure delineating the steps for tissue collection from distinct GBM regions (Core, Rim, and Invasive margin) followed by 5ALA-based FACS isolation of Invasive margin cells into 5ALA + and 5ALA - subpopulations ( A ). The spatially resolved RNA profile (SPRP) from each unsorted region and sorted cells were interrogated by gene set enrichment analysis (GSEA), Deconvolution algorithm, single-cell gene signature scoring (SC sig-scoring), Exon-intron split analysis (EISA), network inference, and complemented by spatially resolved transcriptomics (stRNA-seq) ( A ).

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