Bar Plot from Scientific Research

Quantification of recombination efficiencies in HEK293T cells 48 h after transfection for indicated variants, analyzed by flow cytometry. Recombination rates were calculated as the percentage of the recombined cells (mCherry positive) normalized for transfection efficiency (GFP positive). For f and h , the assay was performed three times ( n = 3, biologically independent samples, plotted as dots). The bar graphs represent mean values, and the error bars indicate the standard deviation from the mean. Statistical relevance was assessed using a two-sample t -test. P values are indicated. ctrl, control.

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