Bar Plot from Scientific Research

Left, top, schematic of truncation–dCas13d fusion used as artificial splicing factor. Left, bottom, schematic of HNRNPD exon 7 used for endogenous splicing modulation, with the position of the two sets of three gRNAs that are co-transfected with the artificial splicing factors as gRNA arrays. Middle, agarose gel showing splicing of HNRNPD exon 7 of a sample replicate for both artificial splicing factors in co-transfection with both gRNA arrays and a non-targeting gRNA (NT). Right, bar graphs displaying quantification of inclusion/exclusion ratio normalized to the non-targeting gRNA (NT) from gels in Extended Data Fig. 6c,d (mean ± s.d., n = 3 replicate transfections). bp, base pairs.

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