Recognition specificity of DT4. Reactivity of DT4 in the presence of CIM of some common anaerobic bacteria in human gut and oral flora and two common aerobic bacteria. The bacteria include FNssp.nucleatum (FN), FNssp.polymorphum(FNP), F. necrophorum(FNE), Bacteroides fragilis(BF); Clostridium difficile(CD), Bacteroides vulgatus(BV), Bacteroides intestinalis(BI), Coprococcus comes(CC), Dorea longicatena(DL), Collinsella aerofaciens(CA), Streptococcus salivarius(SS), Escherichia coli(EC) and Bacillus subtilis(BS). +Heat: CIM additionally heated 90 C for 15 minutes. -Heat: CIM without additional heating step. All DNAzyme assays were conducted at 23 C in 1 SB at E/S=100/1 for 4 hours. M: marker, N: negative control (no reaction control). The uncropped images of the two gels in this figure are provided in Figure S12 (panels E and F, respectively) in the Supporting Information.
