Panel I: Time-dependent fluorescence changes of the G-quadruplex-promoted transcription of the Mango II RNA aptamer by the coupled transcription machineries. Curve (a) represents the integrated transcription cascade shown in (A) in the presence of T7 RNAP (2 U/L) and RNase H (10 U/mL), while curve (b) corresponds to the transcription process driven in the absence of T7 RNAP and RNase H. In all experiments: N5/T5=0.5 M, P6/L5=0.5 M, N6/T6=0.5 M, SP6 RNAP=0.5 U/L, NTPs=1 mM, TO1-biotin=2 M. Panel II: Temporal concentration changes of the Mango II RNA aptamer when driving the G-quadruplex-promoted transcription cascade. Curve (a) represents the concentration changes when using the coupled transcription machinery shown in (A), while curve (b) represents the concentration changes when operating in the absence of T7 RNAP and RNase H. Panel III: Time-dependent transient catalytic rates corresponding to the G-quadruplex-promoted formation of the Mango II RNA aptamer by the cascaded transcription machinery. Curve (a) corresponds to the catalytic rates by the cascaded reaction module shown in (A) and curve (b) represents the catalytic rates in the absence of T7 RNAP and RNase H.
