Line Plot from Scientific Research

Transient formation of the ZnIIPPIX/G-quadruplex using different auxiliary conditions of the dynamic transcription machinery. Panel I Effect of NTPs concentrations: (a) 0.25 mM, (b) 0.50 mM, (c) 1.0 mM. For all curves: T7 RNAP=32 nM (2 U/L), RNase H=0.27 nM (8 U/mL). Panel II Effect of T7 RNAP concentrations: (b) 32 nM, (d) 16 nM, (e) 48 nM. For all curves: RNase H=0.27 nM, NTPs=0.50 mM. Panel III Effect of RNase H concentrations: (b) 0.27 nM, (f) 0.20 nM, (g) 0.33 nM. For all curves: T7 RNAP=32 nM, NTPs=0.50 mM. In all experiments, N1/T1=0.2 M, G1/L1=1.0 M, Sr2+ions=20 mM, ZnIIPPIX=1.0 M.

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