(left) Bacteria-induced pH changes depend on glucose concentration in the medium, ρG. For intermediate values (0.050% w/v≤ρG≤0.200% w/v) the pH decreases and reaches a minimum before recovering. For ρG≥0.225% w/v recovery is not observed. Glucose concentration influences bacterial growth, quantified through turbidity (OD) measurements at 600 nm (right). Culture yield increases with ρG, is maximized at intermediate values, and decreases at higher glucose concentration, possibly due to excessive medium acidification. Data for ρG= 0.175, 0.200, and 0.225% w/v are shown as average of three independent repeats, the remaining points are from a single repeat.
