Core-shell DNA-based particles assemble from cholesterolized (core motifs, CMs) and non-cholesterolized DNA nanostars (inner and outer corona motifs, ICM, and OCM, respectively).[54]CMs, composed of cholesterol-functionalized (orange) and non-functionalized strands (blue), form the hydrophobic particle core. ICMs and OCMs create a stabilizing corona. CMs and ICMs bind through domainsIMandIM*-3m, while ICMs and OCMs attach through -* overhangs. Domain can be used to fluorescently label CMs, by connecting to an Alexa Fluor 594-labeled duplex. When not used, is replaced by a poly-T sequence. Sequences of all strands used in this work and composition of all samples are outlined in TablesS1andS2(Supporting Information), respectively. The relative thickness of core and shell region in the schematics is not in scale.
