Cumulative curves and boxplots (inset) showing the absolute value of exon splicing differences upon PTBP1 knockdown. PTBP1-regulated genes were divided into three groups according to their enrichment in ARTR-seq, including no enrichment (No, 0 enrichment 1), low enrichment (Low, 1 enrichment 2) and high enrichment (High, 2 enrichment). The sample size was labeled below the respective box. P values were determined by the two-tailed Student's t -test of the indicated group versus the 'no enrichment' group.