Bar plots showing the expression of TOP2B (marked with a red box) and adjacent genes (THR and SATB1, marked with green boxes) measured by using qRT-PCR in multiple deletion-induced HCT116 clones (dark orange: D5, A17, D108, D116, and D134) compared with the control clones (gray). The error bars indicate the standard deviation of the results for the mutant clones after multiple replications, and the y axis indicates the mean values of the results. All of the mutant clones had significantly increased TOP2B expression after deletion based on two-sided KS tests (*p < 0.01,*p < 0.05, and NS, not significant).
