Microscopy from Scientific Research

The immune marker CBP60g is highly expressed at early infection stages but downregulated at late stages. Promoter-reporter lines for each immune marker were generated by fusion to a 3xfluorophore possessing a nuclear localization signal (NLS). Left: feature plot of immune markers in pathogen-responsive clusters. Dashed line outlines clusters M1M3. Middle: representative images of immune marker expression at different infection stages. Plants were inoculated as described in (A) and mock images are taken at 24 h. Pictures are maximum projections from confocal z stacks. Right: mean florescence intensity per nucleus was calculated and boxplot shows median with minimum and maximum values (n = 6 images from 3 plants). Different letters indicate statistically significant differences (p < 0.0001, ANOVA with Tukey test). Scale bars: 25 m. All experiments were repeated at least two times with similar results. See also Figure S6.

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