Workflow from Scientific Research
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Functional CRISPR-Cas9 library screening of cell adhesion genes implicated in adhesion of ALL cells to MSCs. NALM6 cells transduced with lentivirus expressing Cas9 and gRNAs that target 285 genes, adhesome (232 genes) and TGF pathway (53 genes), were co-cultured with MSCs, and then Nonadh and Adh NALM6 cells were collected for sequencing and MAGeCK analysis.
#Workflow#Flowchart#Illustration#CRISPR-Cas9#Cell Adhesion#ALL Cells#MSCs#NALM6 Cells#Lentivirus#gRNAs#Adhesome#TGF Pathway#Sequencing#MAGeCK Analysis
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