Microscopy from Scientific Research

HeLa cells were processed as in (A), and an already dividing cell was imaged over time and across multiple z planes using wide-field fluorescence microscopy followed by deconvolution. Images represent frames of progressing cytokinesis over time, showing localization of DENND2B at the cell-cell interface and midbody. The yellow arrow represents the localization of DENND2B at the midbody. The blue arrows represent completed abscission. Scale bars, 10 μm for the low-magnification images and 10 μm for the higher-magnification insets (represented with yellow dotted boxes). Dotted white line marks the periphery of dividing cell. MB, midbody; MBR, midbody remanent.

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